Last data update: 24 January 2024 16:39 CET
Plasmid name: pStart-T2-mXbp1 (LMBP 12621)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p12621.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Mouse X-box binding protein 1 cDNA (Xbp1, D11Ertd39e, TREB5, TREB-5, XBP-1, GeneID 22433) Histidine tag (His-tag); N-terminal Mouse chromosome 11 integration targeting sequence |
Promoter: | - |
Ribosome binding site: |
- |
Terminator: | Escherichia coli rrnB operon T2 terminator Escherichia coli rrnB operon T1 terminator |
Selection marker: | Tetracycline (tet) |
Replicon: | Escherichia coli plasmid p15A origin |
Copy number: | Low copy number |
Host range: | Escherichia coli |
Parental clone: | pStart-T2 |
Further information: | The plasmid was constructed by cloning part of the mouse chromosome 11 gDNA, including the mouse Xbp1 coding sequence, into the pStart-T2 vector. Other name of the plasmid is pStart-T2_Xbp1. |
EMBL Accession number: | - |
Latest sequence update: | 21/10/2021 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr T. Hochepied(1) (2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 NEB Stable |
Host reference: | - |
Cultivation medium: | LB-Lennox + tetracycline (10 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pStart-T2-mXbp1 (LMBP 12621) is available at BCCM/GeneCorner. This plasmid was deposited by Dr T. Hochepied . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.