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GeneCorner plasmid details

Last data update: 09 March 2021 04:23 CET

Plasmid name: pUC57-Seq3-mA20-R579A (LMBP 7167)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p7167.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse tumor necrosis factor, alpha-induced protein 3 cDNA (Tnfaip3, A20, Tnfip3, GeneID 21929); mutated synthetic fragment
Promoter: Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pUC57
Further information: The plasmid was constructed by inserting a synthetic sequence into the EcoRV site of pUC57.
The inserted synthetic sequence contains the mA20 fragment between ZF3 and ZF4 with a N-terminal and C-terminal NheI site and the mutation R579A.
The nucleotide sequence of the pUC57 vector corresponds with the EMBL Nucleotide Sequence Database accession number Y14837.1.
Other name of the plasmid is pUC57-Seq3-R579A.
EMBL Accession number: Y14837.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 09/08/2013
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pUC57-Seq3-mA20-R579A (LMBP 7167) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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