Last data update: 24 January 2024 16:39 CET
Plasmid name: pVL1392-hsGCalpha1H (LMBP 3681)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p3681.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human guanylate cyclase 1 soluble subunit alpha 1 cDNA (GUCY1A1, GC-SA3, GUC1A3, GUCY1A3, sGCA1, GeneID 2982) Histidine tag (His-tag); N-terminal |
| Promoter: | Autographa californica nuclear polyhedrosis virus (AcNPV) polyhedrin promoter (PH) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pVL1392 |
| Further information: | The plasmid was created by isolating the human sGCA1 cDNA from pMa9-hsGCα1 and ligating it with a His-tag and enterokinase cleavage system into the pVL1392 vector. The plasmid is meant for expression in SF9 cells/baculovirus system. The nucleotide sequence of the hsGCA1 cDNA corresponds with the Genbank accession number NNM_000856.5.. Other name of the plasmid is pVL1392-hsGCα1H. |
| EMBL Accession number: | NM_000856.5, view at GenBank |
| Latest sequence update: | 28/06/2012 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr P. Brouckaert(1) (2). It was constructed by Y. Gansemans(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pVL1392-hsGCalpha1H (LMBP 3681) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Brouckaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.