Last data update: 24 January 2024 16:39 CET
Plasmid name: pVL1392-hsGCbeta1E (LMBP 3680)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p3680.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human guanylate cyclase 1 soluble subunit beta 1 cDNA (GUCY1B1, GC-S-beta-1, GC-SB3, GUC1B3, GUCY1B3, sGCB1, GeneID 2983) E-tag; N-terminal |
| Promoter: | Autographa californica nuclear polyhedrosis virus (AcNPV) polyhedrin promoter (PH) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pGEM5-hsGCβ1E; pVL1392 |
| Further information: | The plasmid was created by isolating the human sGCB1 cDNA from pGEM5-hsGCβ1E and ligating it with an E-tag into the pVL1392 vector. The plasmid is meant for expression in SF9 cells/baculovirus system. The nucleotide sequence of the hsGCB1 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number X66533.1. Other name of the plasmid is pVL1392-hsGCβ1E |
| EMBL Accession number: | X66533.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 28/06/2012 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr P. Brouckaert(1) (2). It was constructed by Y. Gansemans(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pVL1392-hsGCbeta1E (LMBP 3680) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Brouckaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.