Last data update: 24 January 2024 16:39 CET
Plasmid name: pVRL2Z (LMBP 12693)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p12693.gb |
| Sequence analysis results Genecorner: |
NGS: f01-gc-may2021.fasta |
| Cloned DNA: |
Acinetobacter calcoaceticus plasmid pWH1277 putative plasmid mobilization genes (mobA/mobL-like) Acinetobacter calcoaceticus plasmid pWH1277 putative plasmid rolling-circle replication gene (repB-like) Acinetobacter calcoaceticus putative nickase cDNA (nickase-like) Acinetobacter calcoaceticus putative toxin cDNA (parE2-like) Acinetobacter calcoaceticus putative antitoxin cDNA (paaA2-like) Escherichia coli DNA-binding transcriptional regulator AraC (araC) |
| Promoter: | Escherichia coli plasmid R388 class 1 integron Pc promoter (PcS) Escherichia coli β-lactamase promoter (amp) Phage T7 gene 10 promoter (T7g10) Escherichia coli L-arabinose operon promoter (araB, araBAD, BAD) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
| Terminator: | Acinetobacter calcoaceticus bidirectional Rho-independent termination signal Escherichia coli rrnB operon T2 terminator Escherichia coli rrnB operon T1 terminator |
| Selection marker: | Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Acinetobacter calcoaceticus plasmid pWH1277 origin |
| Host range: | Escherichia coli Acinetobacter spp. |
| Parental clone: | pVRL1Z |
| Further information: | The plasmid was constructed by PCR amplifying part of the arabinose-inducible araC-pBAD expression cassette, and cloning it into the NsiI/XhoI opened pVRL1Z plasmid. This plasmid is an E. coli-Acinetobacter spp. shuttle vector, intended for gene cloning and arabinose-inducible gene expression in multidrug resistant Acinetobacter species. pVRL2Z is maintained in the absence of antibiotic selection by the presence of a toxin-antitoxin system. |
| EMBL Accession number: | - |
| Latest sequence update: | 03/05/2021 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr. M. Lucidi (1) and Prof. P. Visca (1). (1) Faculty of Science, University of Roma Tre, Rome, Italy |
| Plasmid reference: | Lucidi et al., Antimicrob. Agents Chemother. 62 (2018), e02480-17 [PMID: 29339383] [DOI: 10.1128/AAC.02480-17] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + zeocin (25 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pVRL2Z (LMBP 12693) is available at BCCM/GeneCorner. This plasmid was deposited by Dr. M. Lucidi and Prof. P. Visca and was published in Lucidi et al., 2018. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.