Last data update: 24 January 2024 16:39 CET
Plasmid name: pVSV-MALT1Cd100 (LMBP 8480)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p8480.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human mucosa associated lymphoid tissue lymphoma translocation gene 1 (MALT1); minimal expressing domain Vesicular stomatitis virus glycoprotein (VSV-G) epitope; N-terminal |
Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Escherichia coli lac operon promoter; mutant (lacUV5) Simian virus 40 early promoter (SV40 early) Phage T7 gene 10 promoter (T7g10) Phage SP6 promoter |
Ribosome binding site: |
- |
Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) Bovine growth hormone polyadenylation signal (BGH polyA) |
Selection marker: | Ampicillin (amp) Neomycin (neo; G418) Chloramphenicol (cam) |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pENTR221-MALT1Cd100; pVSV-N-GW |
Further information: | This Gateway expression vector was constructed by cloning the human MALT1 minimal expressing domain from pENTR221-MALT1Cd100 into pVSV-N-GW. |
EMBL Accession number: | - |
Latest sequence update: | 20/08/2013 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pVSV-MALT1Cd100 (LMBP 8480) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.