Last data update: 24 January 2024 16:39 CET
Plasmid name: pYM71P (LMBP 11192)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p11192.gb |
| Sequence analysis results Genecorner: |
NGS: h10-gc-dec2019.fasta |
| Cloned DNA: |
- |
| Promoter: | Candida albicans elongation factor 1-alpha 2 promoter (TEF2) Candida albicans galactokinase 1 promoter (GAL1) |
| Ribosome binding site: |
- |
| Terminator: | Candida albicans ATP-dependent 6-phosphofructokinase subunit beta terminator (PFK2) Candida albicans elongation factor 1-alpha 2 terminator (TEF2) |
| Selection marker: | Ampicillin (amp) Hygromycin (hyg) |
| Replicon: | Escherichia coli plasmid pMB1 origin Candida albicans autonomously replicating sequence ARS |
| Host range: | Escherichia coli Candida albicans |
| Parental clone: | pYM71 |
| Further information: | The plasmid was constructed by replacing the Candida albicans ACT1 terminator downstream of the HygB cassette in pYM71 by a Candida albicans PFK2 terminator. The plasmid can be used for stable expression in Candida albicans under the control of the GAL1 promoter and TEF2 terminator. The nucleotide sequence of the plasmid corresponds with the EMBL Nucleotide Sequence Database accession number MF048863.1. |
| EMBL Accession number: | MF048863.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 26/03/2019 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr K. Ganesan(1). It was constructed by Dr S. Bijlani(1). (1) CSIR Institute of Microbial Technology, Chandigarh, India |
| Plasmid reference: | - |
| Related plasmid reference: | Basso et al., Eukaryot. Cell 9 (2010), 960-970 [PMID: 20348384] [DOI: 10.1128/EC.00355-09] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pYM71P (LMBP 11192) is available at BCCM/GeneCorner. The plasmid was deposited by Dr K. Ganesan. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.