Last data update: 24 January 2024 16:39 CET
Plasmid name: pcDNA3.1-Flag-hMALT1-N-R149Q (LMBP 9269)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9269.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, paracaspase 1); modified fragment FLAG epitope tag; N-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pcDNA3.1-Flag-hMALT1-N |
| Further information: | The plasmid was constructed by introducing an R149Q mutation into the human MALT1 fragment of pcDNA3.1-Flag-hMALT1-N via site-directed mutagenesis. This plasmid contains an N-terminal fragment of human MALT1, encoding the DD-Ig1-Ig2 domains. This fragment acts slightly dominant-negative downstream of CARMA1 overexpression in NF-kB luciferase assays, indicating that it can compete with endogenous MALT1 for critical adaptor proteins. The R149Q mutation corresponds with the human SNP rs147414021, which involves an autoprocessing site in MALT1. The nucleotide sequence of the wild type human MALT1 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF130356.2. Other name of the plasmid is pCDNA3-F-hMALT1-N-R149Q. |
| EMBL Accession number: | AF130356.2, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 12/04/2017 |
| Sequence detail: | Primers used for site-directed mutagenesis in the human MALT1 coding sequence:
MALT1-R149Q-F: 5' AG.TTT.GTG.AAA.CTG.TGT.TGC.CAG.GCA.ACT.GGA.CAT.CCT
^
MALT1-R149Q-R: 5' AGG.ATG.TCC.AGT.TGC.CTG.GCA.ACA.CAG.TTT.CAC.AAA.CT
^
^: mutated nucleotide
Punctuation indicates reading frame. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by DR J. Staal(1)(2) and Prof. Dr R. Beyaert(1)(2). (1) VIB Center for Inflammation Research, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pcDNA3.1-Flag-hMALT1-N-R149Q (LMBP 9269) is available at BCCM/GeneCorner. The plasmid was deposited by DR J. Staal and Prof. Dr R. Beyaert. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.