Last data update: 24 January 2024 16:39 CET
Plasmid name: pSilencer3.1-H1hygro (LMBP 5118)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5118.gb
(View with Genome Compiler) p5118.txt p5118.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd) Influenza HA epitope encoding the haemagglutinin tagging peptide; C-terminal |
| Promoter: | Human H1 RNA polymerase III promoter Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Hygromycin (hyg) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSilencer3.1-H1hygro; pAS2-hABIN1FL |
| Further information: | The vector was created by inserting a 542 bp BamHI-HindIII fragment, containing the GALbd cDNA and the HA epitope tag from pAS2-hABIN1FL, into the BamHI-HindIII opened pSilencer3.1-H1hygro vector. The GALbd-HA-epitope cassette was inserted in reverse orientation relative to the H1 promoter. The purpose of this insert is to ease the isolation of the vector part. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727092.1. The nucleotide sequence of the H1 promoter corresponds with the EMBL Nucleotide Sequence Database accession number X16612.1. |
| EMBL Accession number: | X16612.1, view at EMBL, GenBank, DDBJ LT727092.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 30/03/2009 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI/HindIII, NdeI/SspI, PstI, RsaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed by M. Haegman(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Myslinski et al., Nucleic Acids Res. 29 (2001), 2502-2509 [PMID: 11410657] pSilencer hygro kit manual (Applied Biosystems) |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSilencer3.1-H1hygro (LMBP 5118) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.