GeneCorner plasmid details

Last data update: 20 September 2021 09:16 CEST

Plasmid name: p423MET25 (LMBP 4013)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence:
analysis results


Cloned DNA: -
Promoter: Saccharomyces cerevisiae O-acetyl homoserine sulfhydrylase promoter (MET25)
Escherichia coli lac operon promoter
Phage T3 promoter
Phage T7 gene 10 promoter (T7g10); synthetic sequence
binding site:
Terminator: Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Selection marker: Ampicillin (amp)
HIS3; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Saccharomyces cerevisiae 2 micron plasmid origin (2μ), incl. region conferring stability (STB)
Phage f1 origin
Copy number: High copy number
Host range: Escherichia coli
Saccharomyces cerevisiae; his3(-)
Parental clone: pRS423
Further information: This plasmid is a yeast expression shuttle vector.
The expression from the S. cerevisiae O-acetyl homoserine sulfhydrylase promoter (MET25) is repressed when cells are grown in the presence of methionine.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
Other name of the plasmid is p423 MET25.
EMBL Accession number: -
Latest sequence update: 17/01/2000
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Müller(1).
(1) Philipps University of Marburg, School of Medicine, Institute of Molecular Biology and Tumor Research (IMT) - Center for Tumor Biology and Immunology (ZTI), Marburg, Germany
Plasmid reference: Mumberg et al., Nucleic Acids Res. 22 (1994), 5767-5768 [PMID: 7838736]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB HB101
Host reference: Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022]
Related host reference: Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: ATCC 87319

Refer in your Materials and Methods:

p423MET25 (LMBP 4013) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Müller and was published in Mumberg et al., 1994.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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