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GeneCorner plasmid details

Last data update: 04 December 2020 04:17 CET

Plasmid name: p714 (LMBP 2241)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2241.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli outer membrane protein II gene (ompA); signal sequence
Human β2 microglobulin gene (hB2M); mature sequence
Escherichia coli lac repressor gene (lacI)
Promoter: Escherichia coli lipoprotein promoter (lpp)
Escherichia coli lac operon promoter; mutant (lacUV5)
Escherichia coli lac repressor promoter; mutant (lacI(q))
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: Escherichia coli lipoprotein terminator (lpp)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR322; pIN-II-A1; pIN-III-A3; pIN-III-ompA2
Further information: This plasmid contains the complete signal sequence of the E. coli outer membrane protein II fused in phase to the mature human β2 microglobulin gene.
The nucleotide sequence at the 5' and 3' end of the E. coli lacI insert of the plasmid pLPPsOmpArPDI has been sequenced at Roche Research USA and was kindly provided by Dr. Yves Guisez from Roche Research Ghent. The unknown nucleotides were replaced by the corresponding nucleotides of the E. coli K12 genome sequence obtained from Genbank (Accession number NC_000913.1). Based on these data, the nucleotide sequence of p714 has been adapted.
EMBL Accession number: NC_000913.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 25/11/2003
Sequence detail:
Nucleotide sequence at the fusion between the ompA signal sequence and the hB2M mature sequence:

      ----- ompA signal sequence ----> ----- hB2M mature sequence ---->
5' ... GGT.TTC.GCT.ACC.GTA.GCG.CAG.GCC.ATC.CAG.CGT.ACT.CCA.AAG.ATT.CAG ... 3'
       Gly Phe Ala Thr Val Ala Gln Ala Ile Gln Arg Thr Pro Lys Ile Gln

Punctuation indicates reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K.C. Parker(1).
(1) Harvard University, Cambridge, USA
Plasmid reference: Parker et al., Gene 83 (1989), 117-124 [PMID: 2687112]
Related plasmid reference: Nakamura et al., EMBO J. 1 (1982), 771-775 [PMID: 6329703]
Ghrayeb et al., EMBO J. 3 (1984), 2437-2442 [PMID: 6094184]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 SE6004
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

p714 (LMBP 2241) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K.C. Parker and was published in Parker et al., 1989.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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