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GeneCorner plasmid details

Last data update: 12 April 2021 09:56 CEST

Plasmid name: pACM37 (LMBP 9066)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9066.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Klebsiella oxytoca mercury reductase gene (merA); fragment
Klebsiella oxytoca mercury resistance co-regulator gene (merD)
Klebsiella oxytoca broad-spectrum mercury transporter gene (merE)
Klebsiella oxytoca quaternary ammonium compound resistance protein gene (qacEdelta1)
Klebsiella oxytoca IS6100 transposase gene (tnpA)
Klebsiella oxytoca Urf2Y protein gene (urf2Y)
Promoter: Escherichia coli lac operon promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: -
Selection marker: Ampicillin (amp)
Gentamicin (Gm)
Streptomycin (Sm; spectinomycin (Sp))
Sulphonamide (Sul)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pUC19; pACM1
Further information: The plasmid was constructed by inserting an EcoRI/KpnI fragment of Klebsiella oxytoca plasmid pACM1 in pUC19.
A clone was selected from a library on Mueller-Hinton II agar with 76 μg/ml sulfamethoxazole.
The plasmid contains the complete integron cassettes aacC1, conferring resistance to gentamicin and aadA1, conferring resistance to streptomycin and spectinomycin.
The plasmid further contains the SulI gene, conferring resistance to sulphonamides.
The nucleotide sequence of the parental pACM1 plasmid corresponds with the Genbank accession number KJ541681.1.
EMBL Accession number: KJ541681.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 31/03/2014
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI/EcoRV, KpnI, PstI and PvuII.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K. Preston(1).
(1) Center for Functional Genomics, University at Albany, Rensselaer, NY, USA
Plasmid reference: Preston et al., Plasmid 37 (1997), 105-118 [PMID: 9169202] [DOI: 10.1006/plas.1997.1284]
Related plasmid reference: Preston et al., Plasmid 42 (1999), 92-103 [PMID: 10489326] [DOI: 10.1006/plas.1999.1417]
Preston et al., Plasmid 76 (2014), 54-65 [PMID: 25291385] [DOI: 10.1016/j.plasmid.2014.08.005]
Restricted distribution: - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
- BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pACM37 (LMBP 9066) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. Preston and was published in Preston et al., 1997.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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