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GeneCorner plasmid details

Last data update: 27 November 2020 04:15 CET

Plasmid name: pATHIFNBg42 (LMBP 1655)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1655.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interferon β gene (IFNB, IFNB1)
Promoter: Human interferon β promoter (IFNB)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pATHIFNBg41
Further information: The plasmid was derived from pATHIFNBg41 by deletion of 11 nucleotides, thereby changing the hIFNβ stop codon TGA into the TAG stop codon and destroying the unique BglII site. The plasmid contains the genomic hIFNβ gene with 3' and 5' flanks. See also pATHIFNBg41 for basic constructions.
The tetracycline gene is presumably not biologically active.
Other names of the plasmid are pg4-delB and pAT153gHIFNB2.
EMBL Accession number: -
Latest sequence update: 17/09/1987
Sequence detail:
Nucleotide sequence around the termination codon of hIFNB:

5' GGTTACCTCCGAAACTAG CCTGTGCCTCTGG 3'
   BstEII         *         MnlI

*: Termination codon of the human IFNB gene.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by W. Kempenaers(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pATHIFNBg42 (LMBP 1655) is available at BCCM/GeneCorner. This plasmid was deposited by W. Kempenaers and Prof. Dr E. Remaut.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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