Last data update: 24 January 2024 16:39 CET
Plasmid name: pATHIFNBg42 (LMBP 1655)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p1655.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interferon β gene (IFNB, IFNB1) |
| Promoter: | Human interferon β promoter (IFNB) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pATHIFNBg41 |
| Further information: | The plasmid was derived from pATHIFNBg41 by deletion of 11 nucleotides, thereby changing the hIFNβ stop codon TGA into the TAG stop codon and destroying the unique BglII site. The plasmid contains the genomic hIFNβ gene with 3' and 5' flanks. See also pATHIFNBg41 for basic constructions. The tetracycline gene is presumably not biologically active. Other names of the plasmid are pg4-delB and pAT153gHIFNB2. |
| EMBL Accession number: | - |
| Latest sequence update: | 17/09/1987 |
| Sequence detail: | Nucleotide sequence around the termination codon of hIFNB: 5' GGTTACCTCCGAAACTAG CCTGTGCCTCTGG 3' BstEII * MnlI *: Termination codon of the human IFNB gene. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by W. Kempenaers(1) and Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pATHIFNBg42 (LMBP 1655) is available at BCCM/GeneCorner. This plasmid was deposited by W. Kempenaers and Prof. Dr E. Remaut. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.