GeneCorner plasmid details

Last data update: 20 September 2021 09:16 CEST

Plasmid name: pBRcHIL6f2 (LMBP 1914)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: (View with Genome Compiler)
analysis results


Cloned DNA: Human interleukin 6 cDNA (IL6, IL-6, IFNB2, BSF2, GeneID 3569); fragment
Promoter: -
binding site:
Terminator: -
Selection marker: Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR322
Further information: The plasmid was constructed by inserting a dT-tailed human interleukin 6 cDNA fragment (hIL6f) into the PstI opened, dA-tailed pBR322; although this insert extends 131 bp into the 5' direction further than the corresponding cDNA fragment of pBRcHIL6f1, the coding information for the mature polypeptide is still incomplete.
The length of the natural dA-tail and of the enzymatically dA/dT-tails has not been determined.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727599.1.
Name mentioned in Haegeman et al. (1986) is 26K-2.
EMBL Accession number: LT727599.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 10/04/1989
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AccI and XbaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Haegeman et al., Eur. J. Biochem. 159 (1986), 625-632 [PMID: 3758081]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli
Host reference: -
Cultivation medium: LB-Lennox + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRcHIL6f2 (LMBP 1914) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Haegeman and was published in Haegeman et al., 1986.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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