Last data update: 21 October 2020 04:26 CEST
Plasmid name: pBRcHIL6f2 (LMBP 1914)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||Human interleukin 6 cDNA (IL6); fragment
|Selection marker:||Tetracycline (tet)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||The plasmid was constructed by inserting a dT-tailed human interleukin 6 cDNA fragment (hIL6f) into the PstI opened, dA-tailed pBR322; although this insert extends 131 bp into the 5' direction further than the corresponding cDNA fragment of pBRcHIL6f1, the coding information for the mature polypeptide is still incomplete.
The length of the natural dA-tail and of the enzymatically dA/dT-tails has not been determined.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727599.1.
Name mentioned in Haegeman et al. (1986) is 26K-2.
|EMBL Accession number:||LT727599.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||10/04/1989|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: AccI and XbaI.|
|History of deposit:||This plasmid was deposited by Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Haegeman et al., Eur. J. Biochem. 159 (1986), 625-632 [PMID: 3758081]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli|
|Cultivation medium:||LB-Lennox + tetracycline (10 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBRcHIL6f2 (LMBP 1914) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Haegeman and was published in Haegeman et al., 1986.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.