Last data update: 30 October 2020 04:11 CET
Plasmid name: pBRphoAE6Hf2 (LMBP 2958)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Escherichia coli alkaline phosphatase A gene (phoA); signal sequence
Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; truncated heavy chain of the F(ab')2 fragment E6F2 (E6Hf2)
|Promoter:||Escherichia coli hybrid tryptophan/lacUV5 promoter (tac)|
|Ribosome binding site (RBS) of the Escherichia coli alkaline phosphatase A gene (phoA)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Parental clone:||pBRphoAE6H; pSV25SE6Hf2|
|Further information:||The plasmid was constructed by ligation of three fragments; the large EcoRI-SalI fragment (ori and amp) and the small EcoRI-PvuII fragment (tac promoter and signal sequence of phoA) of pBRphoAE6H, with the PvuII-SalI fragment of pSV25SE6Hf2 (containing the variable domain, constant part 1 and the hinge region of E6H). This plasmid allows the synthesis of a truncated mouse heavy chain with a CH2 of only 8 amino acids .
In this plasmid the E6H gene of pBRphoAE6H was replaced by the E6Hf2 fragment of pSV23SE6Hf2.
The sphoA-E6Hf2 fusion is in-phase.
The presence of the lacI(q) repressor gene is necessary to control the activity of the tac promoter.
The name mentioned in Flamez et al. (1995) is pBRphoAHf2.
|EMBL Accession number:||-|
|Latest sequence update:||04/02/1994|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Dr K. De Sutter(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Flamez et al., J. Biotechnol. 42 (1995), 133-143 [PMID: 7576533]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061F'|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBRphoAE6Hf2 (LMBP 2958) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter and was published in Flamez et al., 1995.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.