Last data update: 24 October 2020 04:12 CEST
Plasmid name: pCAGGS-pIL-1B (LMBP 4441)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||Human interleukin 1β cDNA (IL1B, IL-1B, IL-1BETA)
|Promoter:||Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
|Terminator:||Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
|Host range:||Escherichia coli
Mammalian cells; SV40 permissive cells
|Parental clone:||pCAGGS; 88.YH|
|Further information:||This plasmid was constructed by inserting the EcoRI fragment from the 88.YH vector, containing the human IL1B prosequence cDNA, into the EcoRI opened pCAGGS vector.
pCAGGS-pIL-1B is useful for highly efficient expression of the human IL1β prosequence under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727210.1.
The nucleotide sequence of the human IL1B insert corresponds with the EMBL Nucleotide Sequence Database accession number M15330.1.
Since the nucleotide sequence at the 3' end of the human IL1B insert could not be completely traced back, there is uncertainty as to the cutting frequency of the restriction enzymes, except for the sites that were analysed in the authenticity test.
Other name of the plasmid are pcaggshumanpIL1β#12a and pCAGGS-pro-IL-1beta.
Name mentioned in Kersse et al (2011) is pCAGGS-pro-IL-1B.
|EMBL Accession number:||M15330.1, view at EMBL, GenBank, DDBJ
LT727210.1, view at EMBL, GenBank, DDBJ
|Latest sequence update:||05/11/2013|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: DrdI, HindII, HindII/PvuII, HindIII, SacI and XmnI.|
|History of deposit:||This plasmid was deposited by Prof. Dr P. Vandenabeele(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Van de Craen et al., FEBS Lett. 403 (1997), 61-69 [PMID: 9038361]
|Related plasmid reference:||Kersse et al., J. Biol. Chem. 286 (2011), 35874-35882 [PMID: 21862576] [DOI: 10.1074/jbc.M111.242321]
Weigert et al., Blood 115 (2010), 3531-3540 [PMID: 20197547] [DOI: 10.1182/blood-2009-10-243444]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5α|
|Host reference:||Focus 8 (1986), 9
|Related host reference:||Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pCAGGS-pIL-1B (LMBP 4441) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Vandenabeele and was published in Van de Craen et al., 1997.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.