Last data update: 24 January 2024 16:39 CET
Plasmid name: pHHAM (LMBP 2735)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
HHAM retroviral backbone |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains expressing the lacZ ω fragment (lacZΔM15) for screening |
| Parental clone: | pUC8 |
| Further information: | pHHAM is a handicapped retroviral vector derived from Harvey, Abelson and Moloney mouse retroviruses. The HHAM retroviral backbone (2.2kb fragment) was inserted into the polylinker of pUC8. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. |
| EMBL Accession number: | - |
| Latest sequence update: | 02/03/2005 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr B. Mintz(1). (1) Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA |
| Plasmid reference: | Hawley et al., Proc. Natl. Acad. Sci. U.S.A. 84 (1987), 2406-2410 [PMID: 3031683] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH1 |
| Host reference: | Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] |
| Cultivation medium: | LB-Lennox + ampicillin (25 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pHHAM (LMBP 2735) is available at BCCM/GeneCorner. This plasmid was deposited by Dr B. Mintz and was published in Hawley et al., 1987. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.