Last data update: 24 January 2024 16:39 CET
Plasmid name: pMS27 (LMBP 5349)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5349.gb
(View with Genome Compiler) p5349.txt p5349.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Phage MS2 genome; cDNA Escherichia coli insertion sequence IS1 insB |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBR322; MS2 |
| Further information: | This plasmid contains a nearly full copy (the first 14 nucleotides are missing) of the phage MS2 genome, obtained by cDNA synthesis, and cloned via dT-tailing in the dA-tailed PstI site of pBR322. The exact length of the dA/dT-tails is not known. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727009.1. Name mentioned in Devos et al. (1979) is pMS2-7. |
| EMBL Accession number: | LT727009.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 24/03/1992 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AvrII/SphI, BamHI, BglI/EcoRV, NheI, NheI/PstI, PstI, ScaI and SspI. The sequence of the MS2 cDNA has not been checked (sequenced) on possible mistakes, but the restriction pattern fits the original RNA sequence. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr R. Devos(1) and Prof. Dr W. Fiers(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Devos et al., J. Mol. Biol. 128 (1979), 595-619 [PMID: 374745] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | LMBP 00266 |
Refer in your Materials and Methods: |
pMS27 (LMBP 5349) is available at BCCM/GeneCorner. This plasmid was deposited by Dr R. Devos and Prof. Dr W. Fiers and was published in Devos et al., 1979. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.