Last data update: 06 May 2021 08:34 CEST
Plasmid name: pMS27 (LMBP 5349)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
Phage MS2 genome; cDNA|
Escherichia coli insertion sequence IS1 insB
|Selection marker:||Tetracycline (tet)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Parental clone:||pBR322; MS2|
|Further information:||This plasmid contains a nearly full copy (the first 14 nucleotides are missing) of the phage MS2 genome, obtained by cDNA synthesis, and cloned via dT-tailing in the dA-tailed PstI site of pBR322. The exact length of the dA/dT-tails is not known.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727009.1.
Name mentioned in Devos et al. (1979) is pMS2-7.
|EMBL Accession number:||LT727009.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||24/03/1992|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: AvrII/SphI, BamHI, BglI/EcoRV, NheI, NheI/PstI, PstI, ScaI and SspI.
The sequence of the MS2 cDNA has not been checked (sequenced) on possible mistakes, but the restriction pattern fits the original RNA sequence.
|History of deposit:||This plasmid was deposited by Dr R. Devos(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Devos et al., J. Mol. Biol. 128 (1979), 595-619 [PMID: 374745]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5α|
|Host reference:||Focus 8 (1986), 9
|Related host reference:||Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
|Cultivation medium:||LB-Lennox + tetracycline (10 μg/ml)|
|Other culture collection numbers:||LMBP 00266|
Refer in your Materials and Methods:
|pMS27 (LMBP 5349) is available at BCCM/GeneCorner. This plasmid was deposited by Dr R. Devos and Prof. Dr W. Fiers and was published in Devos et al., 1979.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.