Last data update: 24 January 2024 16:39 CET
Plasmid name: pRM2 (LMBP 4984)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4984.gb
(View with Genome Compiler) p4984.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Candida albicans URA3; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Candida albicans autonomously replicating sequence ARS2 Candida albicans autonomously replicating sequence ARS3 |
| Host range: | Escherichia coli Candida albicans; ura3(-) |
| Parental clone: | pRM1 |
| Further information: | This plasmid was derived from pRM1 by removal of the Esp3I/NruI fragment containing the Candida albicans LEU2 gene. pRM2 is a non-integrative, double-ARS shuttle vector for C. albicans strains. The presence of both C. albicans autonomously replicating sequences ARS2 and ARS3 allows direct recovery in Escherichia coli of non-multimerized plasmids from C. albicans transformants without any intermediate step in Saccharomyces cerevisiae. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727064.1. The nucleotide sequence of the CaURA3 DNA corresponds with the EMBL Nucleotide Sequence Database accession number X14198.1. HOWEVER, THERE MIGHT BE CORRESPONDENCE WITH AF109400.1: see LMBP 4100. |
| EMBL Accession number: | X14198.1, view at EMBL, GenBank, DDBJ LT727064.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 17/01/2002 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AlwNI/NcoI, BamHI, HindII, HindIII, NdeI, PstI, SalI, ScaI and XmnI. The restriction site analysis pattern corresponds to the compiled nucleotide sequence file, with one exception; the results of the HindII digestion do suppose that the plasmid is approx. 100 bp longer. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Janssen Pharmaceutica N.V.(1) and constructed by Dr M. Logghe(2) (3). (1) Beerse, Belgium (2) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (3) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | De Backer et al., Yeast 15 (1999), 1609-1618 [PMID: 10572258] |
| Related plasmid reference: | Johanson et al., J. Mol. Catalysis 59 (2009), 286-291 [DOI: 10.1016/j.molcatb.2008.11.002] Pla et al., Gene 165 (1995), 115-120 [PMID: 7489899] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | LMBP 04346 |
Refer in your Materials and Methods: |
pRM2 (LMBP 4984) is available at BCCM/GeneCorner. This plasmid was deposited by Janssen Pharmaceutica N.V. and constructed by Dr M. Logghe and was published in De Backer et al., 1999. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.