Last data update: 24 January 2024 16:39 CET
Plasmid name: pRM2ScMET15 (LMBP 4490)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4490.gb
(View with Genome Compiler) p4490.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Saccharomyces cerevisiae O-acetylhomoserine O-acetylserine sulfhydrylase gene (ScMET15) |
| Promoter: | Saccharomyces cerevisiae O-acetylhomoserine O-acetylserine sulfhydrylase promoter (ScMET15) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Candida albicans URA3; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Candida albicans autonomously replicating sequence ARS2 Candida albicans autonomously replicating sequence ARS3 |
| Host range: | Escherichia coli Candida albicans; ura3(-) |
| Parental clone: | pRM2; pGC3 |
| Further information: | The plasmid was constructed by inserting the Saccharomyces cerevisiae O-acetylhomoserine O-acetylserine sulfhydrylase gene (ScMET15) as a SpeI fragment of pGC3 (filled in with T4 DNA polymerase) into the SmaI opened pRM2 vector. The presence of both Candida albicans autonomously replicating sequences ARS2 and ARS3 allows direct recovery in Escherichia coli of non-multimerized plasmids from C. albicans transformants without any intermediate step in S. cerevisiae. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727226.1. The nucleotide sequence of the CaURA3 DNA corresponds with the EMBL Nucleotide Sequence Database accession number X14198.1. HOWEVER, THERE MIGHT BE CORRESPONDENCE WITH AF109400.1: see LMBP 4100. |
| EMBL Accession number: | X14198.1, view at EMBL, GenBank, DDBJ LT727226.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 11/03/2002 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HindIII, NsiI, XbaI and XmnI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Contreras(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Viaene et al., Yeast 16 (2000), 1205-1215 [PMID: 10992284] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pRM2ScMET15 (LMBP 4490) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Contreras and was published in Viaene et al., 2000. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.