Last data update: 24 January 2024 16:39 CET
Plasmid name: pROSA26-DV2-hPCDH17-EC-HA (LMBP 12616)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human protocadherin 17 cDNA (PCDH17, PCDH68, PCH68, GeneID 27253); fragment Mouse gene trap ROSA 26 gene (Gtrosa26, Gt(ROSA)26Sor, beta geo, Gtrgeo26, R26, ROSA26, Thumpd3as1, GeneID 14910); 5' UTR and 3' UTR Photinus pyralis (firefly) luciferase gene (LUC); mutated coding region (LUCm; luc(+)) Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP) Escherichia coli lac Z gene (lacZ); with modified 5' end Transposon Tn5 neomycin phosphotransferase gene (neo); modified 5' end |
| Promoter: | Mouse phosphoglycerate kinase 1 promoter (PGK1) Phage T3 promoter Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter Escherichia coli lac operon promoter; mutant (lacUV5) Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only |
| Ribosome binding site: |
Internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) polyprotein gene |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pENTR/D-TOPO-hPCDH17-DN-HA; pROSA26-DV2; pEntry attL4pCAGG-loxP-Bgeo-3xpA-loxP-attL3; pEntry attR2-IRES-eGFP-luc+-pA-attL3 |
| Further information: | This multisite Gateway expression vector was constructed by LR reaction between pROSA26-DV2, pEntry attL4pCAGG-loxP-Bgeo-3xpA-loxP-attL3, pENTR/D-TOPO-hPCDH17-DN-HA and pEntry attR2-IRES-eGFP-luc+-pA-attL3. This plasmid encodes a dominant negative form of human PCDH17. The G4 ROSALUC ES cells (LMBP 10507CB) are available at BCCM/GeneCorner as well. Other name of the plasmid is pROSA26-DV2-hPCDH17-ECdomain-HA cl 41. |
| EMBL Accession number: | - |
| Latest sequence update: | 18/10/2021 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by K. Staes(1)(2) and Prof. Dr F. Van Roy(1)(2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Soriano, Nat. Genet. 21 (1999), 70-71 [PMID: 9916792] Nyabi et al., Nucleic Acids Res. 37 (2009), e55 [PMID: 19279185] [DOI: 10.1093/nar/gkp112] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pROSA26-DV2-hPCDH17-EC-HA (LMBP 12616) is available at BCCM/GeneCorner. The plasmid was deposited by K. Staes and Prof. Dr F. Van Roy. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.