Last data update: 24 January 2024 16:39 CET
Plasmid name: pSCUDT2MFE6H (LMBP 2232)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2232.gb
(View with Genome Compiler) p2232.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Saccharomyces cerevisiae α-mating factor 1 gene (MFα1); prepro secretion signal sequence (ppMF) Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; heavy chain (E6H) missing the leader signal sequence |
| Promoter: | Saccharomyces cerevisiae iso-1-cytochrome C promoter (CYC1) Saccharomyces cerevisiae galactokinase promoter (GAL1) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
| Selection marker: | Ampicillin (amp) Saccharomyces cerevisiae URA3; auxotrophic Methotrexate (MTX) |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-) |
| Parental clone: | pSCUDT2MFE6L; pSCGAL1MFE6H |
| Further information: | The plasmid was constructed by replacing the BamHI fragment of pSCUDT2MFE6L, containing the E6L expression cassette, by the BamHI fragment of pSCGAL1MFE6H, obtained after a partial digestion and containing the complete mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) heavy chain E6H expression cassette (GAL1 promoter, preproMFα1 and mature E6H). The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727633.1. |
| EMBL Accession number: | LT727633.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 10/03/2005 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRV, NcoI/XhoI, SalI, XbaI and XmnI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | .This plasmid was deposited by W. Lammerant(1) (2) and Prof. Dr R. Contreras(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium. |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSCUDT2MFE6H (LMBP 2232) is available at BCCM/GeneCorner. .This plasmid was deposited by W. Lammerant and Prof. Dr R. Contreras . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.