Last data update: 24 January 2024 16:39 CET
Plasmid name: pSOD10 (LMBP 5016)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5016.gb
(View with Genome Compiler) p5016.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Arabidopsis thaliana iron superoxide dismutase cDNA (FeSOD); fragment |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pUC18 |
| Further information: | The plasmid was constructed as follows: 1) a cDNA library from Arabidopsis thaliana was constructed in pUC18; 2) from all SOD containing clones, the one with the largest but still 5'-incomplete A. thaliana iron superoxide dismutase cDNA (FeSOD) insert was selected. The FeSOD insert encodes 23 amino acids at the 5' end, which could be part of a chloroplast transit sequence. Amino acid 24 (asparagine) is presumably the first of the mature protein. Genbank Accession number NM_118642 marks a shorter transit sequence (only 10 codons). When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727070.1. The nucleotide sequence of the FeSOD cDNA corresponds with the Genbank accession number M55910.1. |
| EMBL Accession number: | M55910.1, view at EMBL, GenBank, DDBJ LT727070.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 17/08/2005 |
| Sequence detail: | Sequence at the start of the A. thaliana FeSOD insert:
G-tail |-->presequence
5'... GGGGGGGCC.TCA.CTT.CAC.TCG.TCC.TTG.AAA.CTT.GAG.CTT.CAA.TTA.CAA.
Ser Leu His Ser Ser Leu Lys Leu Glu Leu Gln Leu Gln
|--> mature
| 24 30
AGA.ATG.GCT.GCT.TCA.AGT.GCT.GTC.ACC.GCA.AAC.TAC.GTC.CTC.AAG.CCA.CCT... 3'
Arg Met Ala Ala Ser Ser Ala Val Thr Ala Asn Tyr Val Leu Lys Pro Pro |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: NdeI, PstI and StyI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr D. Inzé(1) (2) and constructed by W. Van Camp(1) (2). (1) Department of Plant Systems Biology, VIB, Ghent, Belgium (2) Department of Plant Systems Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Van Camp et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 9903-9907 [PMID: 2263641] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSOD10 (LMBP 5016) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr D. Inzé and constructed by W. Van Camp and was published in Van Camp et al., 1990. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.