Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV25 (LMBP 1989)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
psv25.gb
(View with Genome Compiler) psv25.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV23 |
| Further information: | pSV25 differs from pSV23 in having a unique E-KpnI site and missing the E-XbaI site. Expression sites: AccI, HindIII, KpnI and SalI which are all unique. Since prokaryotic part is derived from pAT153, there are no 'poison' sequences present. Designed for transient expression in COS cells and constitutive expression in CHO cells (cotransfection with DHFR plasmids). This plasmid does not contain the SV40 small t-antigen splicing signal . It is presumably possible to clone into the unique AvrII and/or unique StuI sites for expression, but this has not been tested. Other name of the plasmid is pSV2-5tss-. |
| EMBL Accession number: | - |
| Latest sequence update: | 08/03/1989 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HindIII and RsaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by D. Huylebroeck(1) and Prof. Dr W. Min Jou(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Danny Huylebroeck (1985) |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV25 (LMBP 1989) is available at BCCM/GeneCorner. This plasmid was deposited by D. Huylebroeck and Prof. Dr W. Min Jou and was published in Danny Huylebroeck, 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.