Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV25S-HTNFR75 (LMBP 2581)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2581.gb
(View with Genome Compiler) p2581.txt p2581.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human tumor necrosis factor receptor p75 cDNA (TNFR75, TNFR2, TNFRSF1B); with Kozak consensus sequence |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV25S; pSPT1hTNFR75 |
| Further information: | The plasmid was constructed as follows: the hTNFR75 coding sequence was inserted as a HindIII-Asp718 (KpnI) fragment from pSPT1hTNFR75 into the unique HindIII and Asp718 (KpnI) sites of pSV25S. The eukaryotic Kozak recognition sequence (GCCGCCACC ATG G) for initiation of translation of hTNFR75 is present. The plasmid contains the SV40 small t-antigen splicing signal. Name of the plasmid in Vandenabeele et al. (1995) is pSV25S-hTNF-R75. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727530.1 The nucleotide sequence of the human TNFR75 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M32315.1. However, due to the PCR amplification for the construction of the parental clone pSPT1hTNFR75, there is one base pair exchange in one codon of the hTNFR75 coding sequence that does not affect the amino acid sequence (silent mutation; W. Lesslauer, F. Hoffmann - La Roche LTD, Basel, Switserland: personal communication). This mutation was not experimentally verified by BCCM/GeneCorner. |
| EMBL Accession number: | M32315.1, view at EMBL, GenBank, DDBJ LT727530.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 30/09/1991 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI/PvuII, BglII/HindIII, EcoRI, HindIII/KpnI and NarI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr P. Vandenabeele(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Vandenabeele et al., J. Exp. Med. 176 (1992), 1015-1024 [PMID: 1328463] |
| Related plasmid reference: | Vandenabeele et al., J. Immunol. 154 (1995), 2904-2913 [PMID: 7876558] Dembic et al., Cytokine 2 (1990), 231-237 [PMID: 1966549] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV25S-HTNFR75 (LMBP 2581) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Vandenabeele and was published in Vandenabeele et al., 1992. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.