Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV51E6Hy3 (LMBP 1982)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p1982.gb
(View with Genome Compiler) p1982.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse anti-hPLAP monoclonal antibody E6(Hy3,κ) half genomic gene; mouse::human chimeric heavy chain (E6Hy3) |
| Promoter: | Simian virus 40 early promoter (SV40 early) Simian virus 40 late promoter (SV40 late) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV51; pBMGNeoE6Hy3 |
| Further information: | The plasmid was constructed by inserting the E6Hy3 gene as a SalI fragment from pBMGNeoE6Hy3 into the SalI vector fragment of pSV51 (nucleotide positions 3095 and 3628). The mouse part of the cloned gene consists of cDNA; the human part consists of genomic DNA. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727611.1. |
| EMBL Accession number: | LT727611.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 05/09/1990 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI/XmnI, EcoRI, EcoRV, PvuI/XbaI, PvuII and SalI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | De Sutter et al., Gene 113 (1992), 223-230 [PMID: 1572543] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12xB HB101 |
| Host reference: | Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022] |
| Related host reference: | Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV51E6Hy3 (LMBP 1982) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers and was published in De Sutter et al., 1992. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.