Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV55i1 (LMBP 2337)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
p2337.gb
(View with Genome Compiler) p2337.pdf |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
- |
Promoter: | Simian virus 40 late promoter (SV40 late) Simian virus 40 early promoter (SV40 early) |
Ribosome binding site: |
- |
Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli; preferably recombination-deficient strains Mammalian cells; SV40 permissive cells |
Parental clone: | pSV55 |
Further information: | This plasmid was constructed as follows: pSV55 was partially BstXI opened (position 4956) and after T4 polymerase (exonuclease) treatment, a BglII linker (CAGATCTG) was ligated. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727501.1. Other name of the plasmid is pSV55B1. |
EMBL Accession number: | LT727501.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 29/04/1991 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglII/EcoRV and BstXI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr P. Vanhoenacker(1) and Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV55i1 (LMBP 2337) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Vanhoenacker and Prof. Dr G. Haegeman. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.