Last data update: 24 January 2024 16:39 CET
Plasmid name: pSVCIFNG (LMBP 2514)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | psvcifng.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Canine interferon γ genomic DNA (IFNG) |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV23 |
| Further information: | This is a recombinant expression vector for canine interferon γ under control of the SV40 early promoter. The genomic canine interferon γ gene was inserted into pSV23 as two fragments: 1) a 750 bp RsaI-EcoRI fragment, with SalI linker ('CGTCGACG') ligated to the blunt RsaI, was isolated from subclone pPL-gcIFN-γa, and was then cloned into the SalI-EcoRI opened pSV23 vector. The sequence of the fragment encompasses the mRNA transcription initiation site up to the EcoRI site located in the first intron. This provided the intermediate construction pSVgcIFN-γ ExI, lacking a SV40 polyadenylation signal. 2) A 5 kb EcoRI fragment, isolated from subclone pgHIFN-γ Eco5, containing exons II, III and IV as well as the polyadenylation signal, was cloned into pSVgcIFN-γ, opened with EcoRI. Biological activity was shown in CHO-transfected cells. Since the nucleotide sequence is not completely known, there is uncertainty as to the cutting frequency of the restriction enzymes indicated. Name mentioned in Devos et al. (1992) is pSVgcIFN-γ. Other name of the plasmid is pSVd2-3gCIFNγ. |
| EMBL Accession number: | - |
| Latest sequence update: | 14/12/1992 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr W. Fiers(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Devos et al., J. Interferon Res. 12 (1992), 95-102 [PMID: 1578190] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSVCIFNG (LMBP 2514) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Devos et al., 1992. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.