Last data update: 24 January 2024 16:39 CET
Plasmid name: pATIPMC (LMBP 1775)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
patipmc.gb
(View with Genome Compiler) patipmc.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Influenza A/Puerto Rico/8/34 matrix cDNA (IPM) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pAT153; pIPM |
| Further information: | The plasmid was constructed by inserting the MspI (HpaII) fragment of pIPM, containing cDNA of the A/Puerto Rico/8/34 influenza matrix gene, into the unique ClaI site of pAT153. The inserted fragment is orientated clockwise. The tetracycline resistance is lost by insertion of the cloned gene into the tetracycline promoter region. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727590.1. Other names of the plasmid are pATM4 and pATMC. |
| EMBL Accession number: | LT727590.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 06/11/1989 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: NcoI, RsaI and StuI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by A. Raeymaekers(1) and Prof. Dr W. Min Jou(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pATIPMC (LMBP 1775) is available at BCCM/GeneCorner. This plasmid was deposited by A. Raeymaekers and Prof. Dr W. Min Jou. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.