Last data update: 20 October 2020 04:14 CEST
Plasmid name: pBRHIG1 (LMBP 1672)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Human immunoglobulin γ1 genomic DNA (hIG1); fragment of the constant portion of the heavy chain
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||The human immunoglobulin γ1 gene encoding the constant domains of the heavy chain (hIG1) was isolated from the partial HaeIII-AluI human genomic library (Maniatis) and subsequently cloned as a HindIII-PvuII fragment of approx. 2770 bp in the HindIII-PvuII opened pBR322 vector.
The 5' flanking region (5FR) is preceding the CH1 exon in the genomic DNA.
pBRHIG1 was designed to add V-domains to the constant portion in an eukaryotic vector, so that it becomes useful in eukaryotic cells.
The nucleotide sequence of hIG1 was taken from Ellison et al. (1982).
Since part of the nucleotide sequence of the 3' flanking region of the hIG1 gene (from nucleotide position 2038 to approx. 2802 in pBRHIG1) is unknown, there is uncertainty as to the cutting frequency of the restriction enzymes indicated.
Other name of the plasmid is pBRHuIG1.
|EMBL Accession number:||-|
|Latest sequence update:||08/05/1992|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Related plasmid reference:||Ellison et al., Nucleic Acids Res. 10 (1982), 4071-4079 [PMID: 6287432]
Takahashi et al., Cell 29 (1982), 671-679 [PMID: 6811139]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12xB HB101|
|Host reference:||Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022]
|Related host reference:||Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBRHIG1 (LMBP 1672) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.