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GeneCorner plasmid details

Last data update: 19 October 2020 04:30 CEST

Plasmid name: pBRSTi163a (LMBP 2031)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2031.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Satellite tobacco necrosis virus (STNV); with insertion
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function
Parental clone: pBRST10
Further information: This plasmid is derived from pBRST10; after a partial RsaI digest (nucleotide position 163) the NcoI-EcoRI linker (TCCATGGGAATTCT) was inserted.
The coat protein reading frame is lost (insert of 14 nucleotides).
Other names of the plasmid are pBRSTNVN160 and pSTBR1l1.
EMBL Accession number: -
Latest sequence update: 15/09/1989
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr P. Ameloot(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRSTi163a (LMBP 2031) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Ameloot and Prof. Dr W. Fiers.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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