Last data update: 24 January 2024 16:39 CET
Plasmid name: pRPhyg (LMBP 4529)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4529.gb
(View with Genome Compiler) p4529.txt p4529.pdf |
| Sequence analysis results Genecorner: |
NGS: a09-gc-dec2018.fasta |
| Cloned DNA: |
BK virus DNA (BKV); fragment of the Dunlop strain |
| Promoter: | Herpes simplex virus (HSV) thymidine kinase promoter (TK) BK virus early promoter (BKV early) BK virus late promoter (BKV late) |
| Ribosome binding site: |
- |
| Terminator: | BK virus polyadenylation signal (BKV polyA) Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) |
| Selection marker: | Ampicillin (amp) Hygromycin (hyg) |
| Replicon: | Escherichia coli plasmid pMB1 origin BK virus origin (BKV) |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pRPneo; p220.2 |
| Further information: | The plasmid was constructed by replacing the neomycin resistance module of the BamHI-opened pRPneo vector by the hygromycin cassette of p220.2. Other name of the plasmid is pRP-hyg. |
| EMBL Accession number: | - |
| Latest sequence update: | 25/04/2005 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, EcoRV, PstI and PvuI. This plasmid has also been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed in the research unit of Prof. Dr G. Haegeman(2) by Dr K. Van Craenenbroeck(2). (1) Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Van Craenenbroeck et al., Gene 253 (2000), 293-301 [PMID: 10940567] |
| Related plasmid reference: | Van Craenenbroeck et al., Eur. J. Biochem. 267 (2000), 5665-5678 [PMID: 10971576] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pRPhyg (LMBP 4529) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert and was published in Van Craenenbroeck et al., 2000. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.