Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV25E6Hy3f2 (LMBP 1685)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p1685.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse anti-hPLAP monoclonal antibody E6(Hy3,κ) half genomic gene; truncated mouse::human chimeric heavy chain of the F(ab')2 fragment E6(Hy3,κ)F2 (E6Hy3f2) |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV25; pATE6Hy3 |
| Further information: | E6Hy3f2 is a HindIII-HindIII fragment of pATE6Hy3; the second HindIII site is made by filling in (Klenow polymerase) the unique MstII site at nucleotide position 1954 of the E6Hy3 gene in pATE6Hy3, and adding at least 2 multi-stop-linkers: 'CTTAGTTAACTAAG'; the succession of the two linkers resulted in the formation of this second HindIII site. The multi- stop-linker contains a HpaI site. This plasmid contains a half-genomic insert encoding the truncated mouse::human chimeric heavy chain of the anti-hPLAP antibody E6(Hy3,κ) clockwise orientated relative to the SV40 early promoter. The mouse part of the cloned gene consists of cDNA; the human part consists of genomic DNA. The plasmid doesn't contain the SV40 small t-antigen splicing signal. The truncated CH2 domain consists of 31 amino acids, terminating at the multi-stop-linker. Designed for transient expression in COS cells or constitutive expression in CHO cells (cotransfection with DHFR plasmids). Other name of the plasmid is pSV25Hy3f2. |
| EMBL Accession number: | - |
| Latest sequence update: | 05/04/1989 |
| Sequence detail: | Terminal sequence of the truncated CH2 domain:
CH2
--------------
Arg Thr Pro Asp Leu Val Asn *
5' CGG ACC CCT GAC TTA GTT AAC TAA GCTTGG 3'
^ HpaI HindIII
------------------
linker
^: 27th amino acid of CH2.
*: Termination codon. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | De Sutter et al., Gene 113 (1992), 223-230 [PMID: 1572543] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12xB HB101 |
| Host reference: | Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022] |
| Related host reference: | Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV25E6Hy3f2 (LMBP 1685) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.