Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV529-hTNF1 (LMBP 2952)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2952.gb
(View with Genome Compiler) p2952.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human tumor necrosis factor cDNA (TNF) |
| Promoter: | Simian virus 40 early promoter (SV40 early) Simian virus 40 late promoter (SV40 late) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli; preferably recombination-deficient strains Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV529; pATHTNF |
| Further information: | The plasmid was constructed as follows: the PstI fragment from pATHTNF, containing the complete coding sequence of the human tumor necrosis factor cDNA (hTNF), was blunted (Klenow exonuclease) and provided with BglII linkers. After treatment with BglII, the fragment was inserted into the unique BamHI site of pSV529. The orientation of the inserted fragment is sense relative to the SV40 late promoter. The plasmid was designed for expression in SV40-permissive cells (CV-1, AP8) and especially in COS. Since 39% of the SV40 genome is duplicated (early region), one should be aware of the possibility of deletions due to homologous recombination. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727561.1. |
| EMBL Accession number: | LT727561.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 27/10/1993 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, NarI, PstI and PvuII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr W. Fiers(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium It was constructed at Roche Research Gent (Gent, Belgium). |
| Plasmid reference: | Marmenout et al., Eur. J. Biochem. 152 (1985), 515-522 [PMID: 3932069] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 SURE |
| Host reference: | Greener, Strategies 3 (1990), 5-6 |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV529-hTNF1 (LMBP 2952) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Marmenout et al., 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.