Last data update: 24 January 2024 16:39 CET
Plasmid name: pSVgMIFNG (LMBP 1433)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p1433.gb
(View with Genome Compiler) p1433.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse interferon γ genomic DNA (IFNG) |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV23; pATgMIFNG |
| Further information: | The BamHI-ClaI fragments from pATgMIFNG were ligated in the unique BamHI and unique ClaI sites of pAT153. This resulted in two intermediate plasmids: pATgMIFNG28 containing exon 2, 3, 4 and the 3' untranslated region of the mIFNG gene (mIFNG) and pATgMIFNG2 containing the mIFNG 1st exon and the 5' untranslated region. In the unique PvuII site of pATgMIFNG2 a HindIII linker was added, resulting in pATgMIFNG2H3. A triple ligation was then realised: the HindIII-ClaI fragment of pATgMIFNG2H3 and the ClaI-BamHI fragment of pATgMIFNG28 were inserted in the HindIII-BamHI opened pSV23 vector. Since the nucleotide sequences of the introns of the mIFNG gene could not be completely traced back, there is uncertainty as to the cutting frequency of the restriction enzymes, except for the sites that were analysed in the authenticity test. The nucleotide sequence of the mouse IFNG cDNA corresponds with the EMBL Nucleotide Sequence Database accession number K00083.1. Other name of the plasmid is pSVgMIFNG6. |
| EMBL Accession number: | K00083.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 07/05/1992 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI/HindIII, EcoRI, HindIII and PstI. The approximative length of the plasmid and of the mIFNG insert deduced from the compiled nucleotide sequence has thus been confirmed. Furthermore, the results revealed that PstI and EcoRI each cut at least 6 times. The locations of the extra PstI and EcoRI sites have not been determined; consequently, the compiled nucleotide sequence has not been adapted. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by F. Duerinck(1) and Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Gray et al., Proc. Natl. Acad. Sci. U.S.A. 80 (1983), 5842-5846 [PMID: 6310596] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSVgMIFNG (LMBP 1433) is available at BCCM/GeneCorner. This plasmid was deposited by F. Duerinck and Prof. Dr E. Remaut. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.