Last data update: 24 January 2024 16:39 CET
Plasmid name: pATHIG32 (LMBP 1676)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | pathig32.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human immunoglobulin γ3 genomic DNA (hIG3); fragment of the constant portion of the heavy chain |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pAT153; pATHIG31 |
| Further information: | The plasmid was constructed as follows: the HaeII site of pATHIG31 (at nucleotide position 1203 and located 7 nucleotides after the polyA signal AATAAA (nucleotide position 2495 of hIG3) was first blunted with T4 DNA polymerase and ligated to the SalI linker 'GGTCGACC'. Then, the HindIII-SalI fragment, encoding the hIG3f gene, was ligated into the HindIII-SalI vector fragment of pAT153. Due to this construction, the tetracycline resistance was lost. Other name of the plasmid is pATHuIG32 !!! |
| EMBL Accession number: | - |
| Latest sequence update: | 05/04/1989 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Huck et al., Nucleic Acids Res. 4 (1986), 1779-1789 [PMID: 3081877] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12xB HB101 |
| Host reference: | Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022] |
| Related host reference: | Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pATHIG32 (LMBP 1676) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.