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GeneCorner plasmid details

Last data update: 25 October 2020 04:11 CET

Plasmid name: pATHIG32 (LMBP 1676)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: pathig32.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human immunoglobulin γ3 genomic DNA (hIG3); fragment of the constant portion of the heavy chain
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pAT153; pATHIG31
Further information: The plasmid was constructed as follows: the HaeII site of pATHIG31 (at nucleotide position 1203 and located 7 nucleotides after the polyA signal AATAAA (nucleotide position 2495 of hIG3) was first blunted with T4 DNA polymerase and ligated to the SalI linker 'GGTCGACC'. Then, the HindIII-SalI fragment, encoding the hIG3f gene, was ligated into the HindIII-SalI vector fragment of pAT153. Due to this construction, the tetracycline resistance was lost.
Other name of the plasmid is pATHuIG32 !!!
EMBL Accession number: -
Latest sequence update: 05/04/1989
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Huck et al., Nucleic Acids Res. 4 (1986), 1779-1789 [PMID: 3081877]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB HB101
Host reference: Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022]
Related host reference: Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pATHIG32 (LMBP 1676) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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