Last data update: 30 July 2021 10:22 CEST
Plasmid name: pATHIG32 (LMBP 1676)
|New search||Print data sheet|
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
Human immunoglobulin γ3 genomic DNA (hIG3); fragment of the constant portion of the heavy chain|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Parental clone:||pAT153; pATHIG31|
|Further information:||The plasmid was constructed as follows: the HaeII site of pATHIG31 (at nucleotide position 1203 and located 7 nucleotides after the polyA signal AATAAA (nucleotide position 2495 of hIG3) was first blunted with T4 DNA polymerase and ligated to the SalI linker 'GGTCGACC'. Then, the HindIII-SalI fragment, encoding the hIG3f gene, was ligated into the HindIII-SalI vector fragment of pAT153. Due to this construction, the tetracycline resistance was lost.
Other name of the plasmid is pATHuIG32 !!!
|EMBL Accession number:||-|
|Latest sequence update:||05/04/1989|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Related plasmid reference:||Huck et al., Nucleic Acids Res. 4 (1986), 1779-1789 [PMID: 3081877]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12xB HB101|
|Host reference:||Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022]
|Related host reference:||Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pATHIG32 (LMBP 1676) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.