SEGA is a standardized genome engineering platform that allows for predictable expression levels and rapid interchange of functional modules. These well-characterized modules were optimized for robust performance in the context of the bacterial genome.
This subset is meant for "Building new functions" according to your own needs.
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You can order strains individually, or order a (sub)set at a strong discount (see price list). |
Add the SEGA subset "Building new functions" to your shopping cart | |
| Strain information can be downloaded for the entire set: | Sequence files (GenBank) |
| Full SEGA set | Subset "Untagged expression" | Subset "J23100" | Subset "PTrc" | Subset "PRha" | Subset "T7" |
| SEGA N° | Accession N° | E. coli strain | Locus | Upstream gadget |
C1 | C2 | Middle gadget |
Cargo | Downstream gadget |
|---|---|---|---|---|---|---|---|---|---|
| SEGA001 | LMBP 12837 | MG1655 | glmS-pstS | none | J23100 | none | none | none | 3'tetA |
| SEGA002 | LMBP 12838 | MG1655 | glmS-pstS | none | Ptrc | none | none | none | 3'tetA |
| SEGA003 | LMBP 12839 | MG1655 | glmS-pstS | none | PrhaBAD | none | none | none | 3'tetA |
| SEGA004 | LMBP 12840 | MG1655(DE3) | glmS-pstS | none | PT7 | none | none | none | 3'tetA |
| SEGA005 | LMBP 12841 | MG1655 | glmS-pstS | none | J23100 | low | BCD | gfp | tetA |
| SEGA006 | LMBP 12842 | MG1655 | glmS-pstS | none | J23100 | medium | BCD | gfp | tetA |
| SEGA007 | LMBP 12843 | MG1655 | glmS-pstS | none | J23100 | high | BCD | gfp | tetA |
| SEGA078 | LMBP 12914 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | low | BCD | gfp | tetA |
| SEGA079 | LMBP 12915 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | medium | BCD | gfp | tetA |
| SEGA080 | LMBP 12916 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | high | BCD | gfp | tetA |
| SEGA081 | LMBP 12917 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | low | Ubiquitin | gfp | tetA |
| SEGA082 | LMBP 12918 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | medium | Ubiquitin | gfp | tetA |
| SEGA083 | LMBP 12919 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | high | Ubiquitin | gfp | tetA |
| SEGA084 | LMBP 12920 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | low | TEV | gfp | tetA |
| SEGA085 | LMBP 12921 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | medium | TEV | gfp | tetA |
| SEGA086 | LMBP 12922 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | high | TEV | gfp | tetA |
| SEGA087 | LMBP 12923 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | low | PROTi | gfp | tetA |
| SEGA088 | LMBP 12924 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | medium | PROTi | gfp | tetA |
| SEGA089 | LMBP 12925 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | high | PROTi | gfp | tetA |
| SEGA090 | LMBP 12926 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | low | PelBss | sfgfp | tetA |
| SEGA091 | LMBP 12927 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | medium | PelBss | sfgfp | tetA |
| SEGA092 | LMBP 12928 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | high | PelBss | sfgfp | tetA |
| SEGA093 | LMBP 12929 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | low | YebF | sfgfp | tetA |
| SEGA094 | LMBP 12930 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | medium | YebF | sfgfp | tetA |
| SEGA095 | LMBP 12931 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | high | YebF | sfgfp | tetA |