SEGA is a standardized genome engineering platform that allows for predictable expression levels and rapid interchange of functional modules. These well-characterized modules were optimized for robust performance in the context of the bacterial genome.
The subset "Untagged expression" allows for genomic modification without upstream tags.
You can order strains individually, or order a (sub)set at a strong discount (see price list). |
Add the SEGA subset "Untagged expression" to your shopping cart | |
Strain information can be downloaded for the entire set: | Sequence files (GenBank) |
Full SEGA set | Subset "Building new functions" | Subset "J23100" | Subset "PTrc" | Subset "PRha" | Subset "T7" |
SEGA N° | Accession N° | E. coli strain | Locus | Upstream gadget |
C1 | C2 | Middle gadget |
Cargo | Downstream gadget |
---|---|---|---|---|---|---|---|---|---|
SEGA001 | LMBP 12837 | MG1655 | glmS-pstS | none | J23100 | none | none | none | 3'tetA |
SEGA002 | LMBP 12838 | MG1655 | glmS-pstS | none | Ptrc | none | none | none | 3'tetA |
SEGA003 | LMBP 12839 | MG1655 | glmS-pstS | none | PrhaBAD | none | none | none | 3'tetA |
SEGA004 | LMBP 12840 | MG1655(DE3) | glmS-pstS | none | PT7 | none | none | none | 3'tetA |
SEGA005 | LMBP 12841 | MG1655 | glmS-pstS | none | J23100 | low | BCD | gfp | tetA |
SEGA006 | LMBP 12842 | MG1655 | glmS-pstS | none | J23100 | medium | BCD | gfp | tetA |
SEGA007 | LMBP 12843 | MG1655 | glmS-pstS | none | J23100 | high | BCD | gfp | tetA |
SEGA020 | LMBP 12856 | MG1655 | glmS-pstS | none | Ptrc | low | BCD | gfp | tetA |
SEGA021 | LMBP 12857 | MG1655 | glmS-pstS | none | Ptrc | medium | BCD | gfp | tetA |
SEGA022 | LMBP 12858 | MG1655 | glmS-pstS | none | Ptrc | high | BCD | gfp | tetA |
SEGA038 | LMBP 12874 | MG1655 | glmS-pstS | none | PrhaBAD | low | BCD | gfp | tetA |
SEGA039 | LMBP 12875 | MG1655 | glmS-pstS | none | PrhaBAD | medium | BCD | gfp | tetA |
SEGA040 | LMBP 12876 | MG1655 | glmS-pstS | none | PrhaBAD | high | BCD | gfp | tetA |
SEGA056 | LMBP 12892 | MG1655(DE3) | glmS-pstS | none | PT7 | low | BCD | gfp | tetA |
SEGA057 | LMBP 12893 | MG1655(DE3) | glmS-pstS | none | PT7 | medium | BCD | gfp | tetA |
SEGA058 | LMBP 12894 | MG1655(DE3) | glmS-pstS | none | PT7 | high | BCD | gfp | tetA |
SEGA074 | LMBP 12910 | MG1655 | glmS-pstS | none | PsaI-RiboJ | low | BCD | gfp | tetA |
SEGA075 | LMBP 12911 | MG1655 | glmS-pstS | none | PsaI-RiboJ | high | BCD | gfp | tetA |
SEGA076 | LMBP 12912 | MG1655 | glmS-pstS | none | PsaI-RiboJ | low | BCD | gfp | tetA |
SEGA077 | LMBP 12913 | MG1655 | glmS-pstS | none | PsaI-RiboJ | high | BCD | gfp | tetA |
SEGA078 | LMBP 12914 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | low | BCD | gfp | tetA |
SEGA079 | LMBP 12915 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | medium | BCD | gfp | tetA |
SEGA080 | LMBP 12916 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | high | BCD | gfp | tetA |
SEGA096 | LMBP 12932 | MG1655 | glmS-pstS | none | J23100 | low | BCD | none | 3'tetA |
SEGA097 | LMBP 12933 | MG1655 | glmS-pstS | none | J23100 | high | BCD | none | 3'tetA |
SEGA098 | LMBP 12934 | MG1655 | glmS-pstS | none | J23100 | high | BCD | gfp | 3'tetA |
SEGA099 | LMBP 12935 | MG1655 | glmS-pstS | none | Ptrc | low | BCD | none | 3'tetA |
SEGA100 | LMBP 12936 | MG1655 | glmS-pstS | none | Ptrc | high | BCD | none | 3'tetA |
SEGA101 | LMBP 12937 | MG1655 | glmS-pstS | none | PrhaBAD | low | BCD | none | 3'tetA |
SEGA102 | LMBP 12938 | MG1655 | glmS-pstS | none | PrhaBAD | high | BCD | none | 3'tetA |
SEGA103 | LMBP 12939 | MG1655(DE3) | glmS-pstS | none | PT7 | low | BCD | none | 3'tetA |
SEGA104 | LMBP 12940 | MG1655(DE3) | glmS-pstS | none | PT7 | high | BCD | none | 3'tetA |